Crystallography on a chip.

نویسندگان

  • Arash Zarrine-Afsar
  • Thomas R M Barends
  • Christina Müller
  • Martin R Fuchs
  • Lukas Lomb
  • Ilme Schlichting
  • R J Dwayne Miller
چکیده

A new chip-based crystal-mounting approach for rapid room-temperature data collection from numerous crystals is described. This work was motivated by the recent development of X-ray free-electron lasers. These novel sources deliver very intense femtosecond X-ray pulses that promise to yield high-resolution diffraction data of nanocrystals before their destruction by radiation damage. Thus, the concept of `diffraction before destruction' requires rapid replenishment of the sample for each exposure. The chip promotes the self-assembly of an array of protein crystals on a surface. Rough features on the surface cause the crystals to adopt random orientations, allowing efficient sampling of reciprocal space.

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عنوان ژورنال:
  • Acta crystallographica. Section D, Biological crystallography

دوره 68 Pt 3  شماره 

صفحات  -

تاریخ انتشار 2012